Emilia Betty Au-Yeung

4 Questions 6 Answers 0 Followers

Questions related from Emilia Betty Au-Yeung

Are constitutive promoters in Staphylococcus aureus also applicable for S epidermidis?

I found that there are many well studied S aureus promoters, I am wondering if promoters like Cap1A or P1 promoters can also be used in S epidermidis?

04 January 2021 2,938 2 View

Can anything replace NH4Cl to quench free aldehyde group from cell fixation?

I'm going to do immunofluorescence staining to check phage entry into cells. The protocol says cells have to be treated with 50mM ammonium chloride to quench free aldehyde groups from fixation...

25 November 2020 3,124 5 View

Can antibody carrying HRP be detected by secondary antibody?

I saw that HRP is attached to the Fc region of the primary antibody, while secondary antibody detects the Fc region of the primary antibody. Will the detection be interfered if the primary...

25 November 2020 7,847 11 View

How do I obtain the nucleotide sequence from amino acid sequence?

I'm trying to figure out the nucleotide sequencing coding for RGD-4C (ACDCRGDCFCG). I couldn't find it in papers... Does anyone know what are the exact nucleotides coding for this peptide? Thank you!

16 August 2020 2,217 3 View