Emilia Zicca

4 Questions 7 Answers 0 Followers

Questions related from Emilia Zicca

Does Pfu Taq polymerase generate 5'P end?

Does the PCR product amplified using pfu polymerase have 5' phosphate groups to use for blunt cloning?

23 June 2016 1,164 3 View

How can I break the exopolysaccharide capsule of the mucous colonies to extract the RNA and DNA?

Hello to everyone! I'm working with strains of Acinetobacter baumannii resuspended in NaCl 0.45% (8 McF in 2 mL). Colonies of Acinetobacter baumannii are mucous so that they can not be separated...

16 June 2016 6,606 1 View

Why do I have a smear in gel for my bacterial RNA sample?

I have a question.....i have made a agarose gel of bacterial RNA and i have obtained a smear....what's happened? the RNA is 588mer. Moreover, i have done a Real Time PCR of these RNA without...

26 October 2015 7,311 4 View

How do I perform a PCR with long primers?

I need to know the concentrations of 66 nt long primers for a PCR reaction and if there are special conditions to be followed

03 March 2015 5,966 11 View