Emil Nielsen

3 Questions 3 Answers 0 Followers

Questions related from Emil Nielsen

How come I get an inverse correlation signal output in my ELISA titration?

Hello, I am identifying nanobodies for my protein of interest. I have identified several different nanobodies showing specificity towards my target and conducted a validation assay (ELISA...

02 December 2023 6,881 1 View

Mini prep plasmid with DNA contamination? How can I avoid DNA contamination trying to QuikChange mutate my plasmid?

Hello, I am currently working on making mutations in my plasmid conducting Quikchange. The plasmid size is 3800bp and I am using primers with several mutations. However, both my parental plasmid...

20 July 2023 5,156 3 View

What is the negative controls in phage display (biopanning) and troubleshooting panning protocol?

Hi there, I am currently panning against GFP and pre-depleting with PBS+0.5%BSA using the steps attached (modified from Barbas's protocol). GFP and BSA are immobilised on high-binding microtiter...

25 January 2023 388 3 View