Does anyone have experience to calculate the titer of dengue virus type 1 in BHK-21 cells?

I'm having a problem with this model because the virus isn't causing plaque lysis in the cell monolayer. To titrate, I'm using a semi-solid medium (carboxymethyl cellulose 1,5% plus 1,5% of FBS)...

29 May 2015 4,813 4 View