Eman Ali

6 Questions 17 Answers 0 Followers

Questions related from Eman Ali

Did I lost peptides by using cut off membrane?

Hello, I used 3 KD cut-off membrane as protein concentrator before mass spec , So what is the possibility to loss peptides in the membrane in comparison with size exclusion chromatography,...

25 April 2017 5,599 7 View

Comparative analysis of the proteolytic systems at a genome scale ?

Hello, I did complete genome sequencing of lactobacillus strain then Comparative analysis of the proteolytic systems at a genome scale with other related genomes , I got the percent of identity of...

25 April 2017 9,965 3 View

Proteinase specific activity calculation???

please I want to know if my calculation is true or not my enzyme assay consist of To 200 μL resuspended cells (sample), added 287.5 μL phosphate buffer ,225 μL 5M NaCl, and 37.5 μL...

08 February 2017 6,503 5 View

Lactobacillus enzyme assay ?

Please ,I work with fermentation of whey isolate with lactobacillus strains ,I would like to measure the cell lysis rate depending on releasing of intracellular peptidase , So I have to measure...

30 January 2017 3,717 5 View

Please how can calculate the protein percent on my sample using biorad assay and specific enzyme activity?

I used stock BSA 2mg/ml, the standard were 2,1,0.8,0.6,0.4,0.2 in 20µl plus 1 ml diluted dye,I did the standard curve but I am confused did the protein value for the standard would be per ml or...

30 December 2016 926 11 View

Can l use the enzyme substrate to make standard curve instead of the product also how can calculate the enzyme activity depending on it?

Thanks in advance

23 July 2016 6,613 8 View