Emadodin Tohidi | Shiraz University

Emadodin Tohidi

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Questions related from Emadodin Tohidi

I have constucted a plasmid vector from puc19 with no antibiotic and lacz gene. The problem is screening for transformed cells. Any suggestions?

Get too many colonies, which are mostly non-transformants when tested by colony PCR

12 July 2024 6,971 3 View

Can one hope that heat-treated single-stranded DNA plasmids, extracted from agarose gel, could replicate within transformed bacterial cells?

Could the lower transformation efficency be problematic in practice?

21 July 2023 3,545 3 View