What can I do to have pcr products with A-T rich sequences using genomic DNA as template?

I have six constructs with the following G-C contents: 29.6%, 23.2%, 27.2%, 24.4%, 27% and 28%. Due to the low G-C content of genes of interest, the Tm of my primers (see attached file) are low;...

04 March 2015 9,113 9 View