Why is absorbance measured at 540 nm instead of at 280 nm when quantifying proteins in Lowry and Buiret methods?

Proteins generally absorb UV light at 280 nm while peptide bonds absorb UV light at 214 nm. When quantifying proteins using the Lowry and Buiret methods, absorbance or optical density is measured...

17 December 2020 1,268 3 View