Elvira Mass

3 Questions 4 Answers 0 Followers

Questions related from Elvira Mass

Why do I only have Bovine proteins in my SILAC analysis?

I did my SILAC experiment (Lys8/Arg10) with bone marrow derived macrophages (cells were cultured for 4 days in light or heavy medium). My cell numbers were quite low (100 000 cells), but I could...

04 April 2015 5,033 3 View

What is the best vector for a TetON system with a bidirectional promoter?

I cloned 2 genes of my interest into the pBI-4 vector, so that I could activate the expression of both genes at the same time. However, the construct is quite leaky, so that I get expression of...

06 June 2014 9,419 2 View

What is the best way to transfect HUVECs?

I tried Metafectene, peqFECT and Fugene. Nothing worked so far.

10 October 2013 5,076 11 View