Elliot Macleod

2 Questions 4 Answers 0 Followers

Questions related from Elliot Macleod

Could my secondary antibody recognise coating antigen in an ELISA?

Hi all, I'm doing an ELISA using an anti-c-myc HRP as the secondary antibody. It recognises the amino acid sequence EQKLISEEDL on my primary antibody. I am getting quite a high background from...

17 March 2019 8,205 5 View

Can TGF-beta reassociate with latency associated protein (LAP)?

As I understand it, TGF-beta is held inactive by non-covalent interactions with LAP until it is released. Is there scope for free LAP to re-associate with free-TGF-beta?

23 November 2017 6,302 0 View