I am having trouble optimizing the Mn-Phos-Tag system for whole cell lysate western blots. Has anyone else had any luck with these gels?

My lab has purchased some of the Super Sep precast Phos-Tag gels from Wako (#198-17981) to try and determine the phosphorylation state of a kinase we're interested in. We have had very little luck...

03 March 2018 6,752 1 View