Elizabeth Regedanz

2 Questions 2 Answers 0 Followers

Questions related from Elizabeth Regedanz

Vector backbone alone eliciting a response in plant pathogen defense?

My colleague is trying to study the effects of a protein on pathogen inhibition in N. benthamiana. He is expressing it in either a pSITE vector or pCAMBIA1302 that had the GFP gene removed. They...

08 January 2024 3,297 1 View

Sample proteins not separating in resolving gel but the ladder does?

I have tried running my samples on a 10% separating gel after heating them for 5 minutes at 98C and placing them on ice. They were heated in standard laemmli buffer. The ladder separates fine, but...

03 January 2024 6,059 4 View