Should I be seeing a yellow color after solubilizing formazan crystals in MTT assay?

Hi everyone,  I am very new to MTT assay and I find myself stuck.  I plated 5,000 cells/well in a 96-well plate. Following recovery and incubation, I added 50uL MTT reagent to each well and...

25 January 2016 4,445 5 View