Elizabeth Berry

3 Questions 2 Answers 0 Followers

Questions related from Elizabeth Berry

Has anyone had any luck using ShuTu neuronal reconstruction software for reconstruction of soma and dendrites?

I am looking for the best user friendly software to reconstruct neurons that were filled with fluorescent dye during whole cell patch clamp recording. Looking into ShutTu software-- does anyone...

02 June 2021 1,804 0 View

What are your protocols for using Vector VIP to stain patched neurons in thick brains sections nominally 250um thick?

Brain section 250um thick patched neurons containing biocytin do you use Triton X 100 with the ABC reaction? Alcohol steps and Xylene post VIP reaction?

22 June 2020 3,680 0 View

How to prevent RNA from sticking to the outside of the patch pipette for neuronal cytoplasm collection following patch recordings

We are patching neurons followed with extracting their cytoplasm to do single cell rt-pcr analysis. The controls we do are to stick the patch pipette into the tissue, to mimic approaching a...

15 August 2017 3,133 2 View