Elise Bonnet

2 Questions 2 Answers 0 Followers

Questions related from Elise Bonnet

How can I increase my transcribed RNA concentration?

I am struggling to get a high enough RNA concentration from transcribed RNA using Invitrogen's MegaScript Sp6 kit. I use Promega's SV total RNA isolation and purification kit to clean up the...

17 August 2018 6,203 4 View

I am designing RPA primers, however I am struggling with multiple wobbles bases (I am using the most conserved gene). Please help?

I am using the most conserved gene (NS5) of flaviviruses. There are multiple wobble bases in potential primer sequences I am looking at, app. 10 at the most. Are there any rules concerning wobble...

13 August 2017 6,227 0 View