Elisabetta Punzi

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Questions related from Elisabetta Punzi

Has any of you encountered issues with protein production in a E.coli cell-free protein expression system?

I'm having trouble synthesizing a mollusk 173 aminoacids protein of unknown function in a E.coli cell-free protein expression system. The positive control works well and the plasmid has been...

02 October 2019 8,879 7 View

Can I freeze a tissue for subsequent Co-IP or do I necessarily need to flash-freeze it?

I'm sampling tissues on which I want to perform co-immunoprecipitation. Currently, I do not have any liquid nitrogen available. Can I freeze my tissue samples simply by storing them in a -80°C...

28 August 2018 8,291 4 View