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Questions related from Elisabeth Billard
Hi everybody, do you know if there is a way of counting archaea suspensions grown in vitro? The suspensions are rather concentrated so counting on filters is not so easy. I'm trying to stain them...
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Dear everybody, I recently started mating Ly5.1 and OT-II mice and I need to genotype the offspring. I intend to stain blood lymphocytes with anti-Ly5.1 and Ly5.2 to identify Ly5.1 homozygous...
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If anyone has already performed a fac analysis of cell suspensions from mice mesenteric lympho nodes, could you please tell me what you think about the dot plots I have attached here? I crushed...
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I would like to perform MLR reactions with DCs and autologous naive T cells, in both human and murine models. In autologous MLRs with antigen-loaded DCs, do I need to put some anti-CD3/CD28 and do...
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I am trying to prepare mononuclear cells from mouse colon lamina propria. I tried either with classical enzymatic digestion (by removing epithelial cells with EDTA and shaking, and then digesting...
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