Elisabeth Spiritosanto

2 Questions 2 Answers 0 Followers

Questions related from Elisabeth Spiritosanto

Image analysis with Fiji: how can I extract the green signal which overlaps with the red signal?

Hi everyone, I am working with cells labelled with Mcherry (membrane) and EGFP (cytoplasm). Following my experiment, I want to quantify the green signal which relocated to the membrane. How can I...

07 June 2021 1,735 3 View

How long should I incubate primary cells with EdU for proliferation assay?

Hello everyone! I have started a primary cell culture from murine heart, bladder and tight. I need to do a proliferation assay using EdU-Click kit, and I was wondering how long should I incubate...

13 September 2019 9,246 1 View