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Questions related from Elisabeth Billard
Hello, I'm staining thawed human PBMC with a Th panel (live/dead, CD3, CD4, CD25, CD127, CXCR3, CCR4, CCR6, CCR10). I usually gate live cells > CD3+ CD4+ T cells > CD25+ CD127- Tregs, then I...
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Hi everybody, do you know if there is a way of counting archaea suspensions grown in vitro? The suspensions are rather concentrated so counting on filters is not so easy. I'm trying to stain them...
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What do you think it means when a treatment reduces the size of MLNs and PPs (I can see it macroscopically and I quantified the change by crushing them and counting the cells)? Do you think it...
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I would like to perform MLR reactions with DCs and autologous naive T cells, in both human and murine models. In autologous MLRs with antigen-loaded DCs, do I need to put some anti-CD3/CD28 and do...
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I am trying to prepare mononuclear cells from mouse colon lamina propria. I tried either with classical enzymatic digestion (by removing epithelial cells with EDTA and shaking, and then digesting...
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