Elisa Milano

3 Questions 11 Answers 0 Followers

Questions related from Elisa Milano

Why do I obtain wavy bands on acrylammide precast gel in western blot experiments?

Hello! I recently tested a new precast gradiented gel for my Western Blot experiments and I obtained wavy bands both for proteins and for molecular weight marker. I run in tris-glycine-sds running...

07 July 2019 4,554 7 View

Tris HCl 1.5M pH 8.8, Tris HCl 1 M pH 6.8, how to pH?

I am experiencing many problems in obtaining the correct pH of Tris-HCl solutions for acrylammide gels (pH8.8 and 6.8) due to the dependence of the pH on temperature. I prepared and brought the...

06 June 2019 3,716 10 View

Why do plasmid digestion gives a smear?

Hi!! I digested 2 micrograms of the PMIY vector with 12 units of EcoRI and XhoI enzymes to get the 500bp insert that I cloned inside, and even if I can visualize the fragment I also observe a...

05 May 2019 4,524 7 View