Any suggestions of a good protocol to isolate both mouse leukocytes and granulocytes from a mixture of fresh isolated immune cells?

I used to isolate mouse leukocytes only by using a 40%-80% percoll gradient (550g for 20 min at RT). This technique was very effective to obtain a good and clean leukocyte population to stain by...

08 August 2014 9,309 6 View