I'm working with mouse embryonic fibroblast, and I'm interested in detecting lysosomal activity through using different assays. Any ideas?

I have a problem when I try to detect Cathpesines B,C and D by WB, I don't know why, maybe it is the cell model. On the other hand, I tested different lysosensors by IF and flow cytometry, but...

12 November 2013 8,248 9 View