Elif Sanli

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Questions related from Elif Sanli

Is MultiScribe reverse transcriptase used instead of SuperScript II reverse transcriptase for cDNA synthesis from miRNA?

Hi everyone! The enzyme in my SuperScript™ II Reverse Transcriptase (Invitrogen) kit has run out and I don't have time to order a new one as I have to finish the experiments asap. Will I run into...

19 August 2021 1,706 3 View

How can I increase the number of CD25+CD49d– Treg cells isolated from frozen PBMCs?

Hi everyone! I have trouble isolating frozen PBMCs with CD25+CD49d– Regulatory T Cell Isolation Kit, human (Miltenyi, # 130-094-551). It is recommended to work with fresh cells, but I have a...

04 March 2021 5,797 2 View