How can I increase the number of CD25+CD49d– Treg cells isolated from frozen PBMCs?

Hi everyone! I have trouble isolating frozen PBMCs with CD25+CD49d– Regulatory T Cell Isolation Kit, human (Miltenyi, # 130-094-551). It is recommended to work with fresh cells, but I have a...

04 March 2021 5,713 2 View