Elif Öztemiz

3 Questions 5 Answers 0 Followers

Questions related from Elif Öztemiz

How Can I Prevent Clustering of Caco-2 Cells Without Using Trypsin?

Dear all, I am currently working with Caco-2 cells and need to culture them to achieve high cell numbers. However, due to my protocol, I cannot use trypsin for detachment. My main challenge is...

21 February 2025 5,332 1 View

Do repeated fluorescent (pHrodo) measurements from the same cells affect signal?

I will measure the internalization of proteins using pHrodo in a time-dependent manner. However, i have limited protein. Therefore, I would like to measure the track the same cells at 5-6 time...

10 October 2024 7,359 3 View

Can I store CaseinFITC (dissolved in water) at -80?

I want to dissolve caseinFITC (commercial) in ddH2O and store it at -80 to have standard aliquotes (instead of weighing powder each time which is prone to mistakes). Literature says unconjugated...

26 July 2024 3,962 5 View