Has anyone had problems with multiple insertions of Mariner based Himar-1 transposons?

Used conjugation to introduce pBT20 (Himar-1 bearing GentR marker) to betaproteobacteria. Conducted a Southern of digested gDNA and got single bands (I think...it's kind of a crappy Southern, but...

11 November 2013 2,168 0 View