Eli Farber

2 Questions 3 Answers 0 Followers

Questions related from Eli Farber

How to grind your tissue for RNA extraction?

Hi fellows, I've started a project in a new lab where I have to extract RNA from mice liver, brain, and muscles. Upon dissection I immediately snap-freeze the tissue in liquid nitrogen and store...

20 February 2024 1,619 4 View

What wet transfer conditions would be best suitable for a 5kDa protein (Aß42) in Western Blot onto a PVDF membrane?

Hi everyone, I've tried to transfer initially using a 20% methanol transfer buffer using 250mA max current for 70 mins, all I got was transfer of mid-high range proteins, I suppose all proteins...

26 July 2023 1,840 4 View