Elham Radvar

3 Questions 2 Answers 0 Followers

Questions related from Elham Radvar

Is there any general protocol for immunohistology staining?

I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?

18 February 2021 3,319 4 View

Why my E. Coli culture has a sharp smell and no protein expressed?

I have been trying to express a protein using E. Coli strains (Rosetta and BL21) and am using LB and TB media. But both cultures have a very sharp acidic smell. I have tried for two weeks to...

10 December 2020 5,267 1 View

How can I remove the protecting group of a thiol (trt) in a cysteine amino acid?

I want to do michael addition of a diacrylate with a cysteine, but I need to deprotect the thiol, which is protected with trt group.

27 February 2015 2,587 3 View