Elena Pomari

3 Questions 3 Answers 0 Followers

Questions related from Elena Pomari

Difference between Digital PCR and Droplet Digital PCR ? Pros and Cons ?

I'm going to perform gene expression analysis by digital system .. which platform should I choose ?

10 October 2018 8,029 0 View

Does anybody know Ct value of human RNU6 using Taqman assay ?

I used U6 as reference and I got a range 29-30 Ct. I found that U6 is 'moderately' expressed. What is its expected ct value ?

02 February 2015 3,531 0 View

Has anybody isolated preadipocytes from frozen tissue ?

Tissues were stored in DMEM + 1% penicillin/streptomicin + FBS 20% + DMSO 10% I thawed tissue quickly and treated with collagenase IA 125U 37 °C 1h. I filtered the tissue and centrifuge at 750 g...

11 November 2014 2,432 8 View