Why have I in my immunohistochemstry using avidin-biotin(ABC) plus AEC substrate a red background staining on the glass of my superfrost plus slides?

I am performing Immunohistochemistry using superfrost plus slides (all my sections are already mounted, because I needed them also for In Situ Hybridisation). Arround the tissue (mostly not on...

25 August 2023 216 1 View