Why after cloning recombinant pET in E. coli DH5a I get a very low plasmid yield after extraction?

I chemically transformed a ligation product (pET28a and my gene of 3051 bp) into E coli DH5alpha competent cells. I plated 200 and 800 ml of my transformation on plates of LB agar Kan (25 mg/ml)....

14 September 2020 4,568 3 View