Elena Demyanova

3 Questions 3 Answers 0 Followers

Questions related from Elena Demyanova

What software you use for visualisation can be used in media and feed optimisation?

Hello! I need visualisation schemes and results (titre, VCD) in media and feed optimisation, because now I have a lot of long schemes in Excel.

15 December 2023 8,916 0 View

Why recombinant protein fused to ABD domain degraded into 2 parts?

Our recombinant protein fused to ABD (albumin binding domain) degraded before and after purification (imax, ABD-agarose) into 2 parts. Protease inhibitors didn’t help. We use linker (G4S)3.

19 October 2023 4,541 2 View

How to grow cells with ccdB in chromosome without plasmid with ccdA?

Hello! I’m going to work with toxin/antitoxin selection system (eg StabyClone). I want to prepare electrocompetent cells with ccdB gene in genome.

21 March 2023 2,607 3 View