Elena Bolonova

2 Questions 4 Answers 0 Followers

Questions related from Elena Bolonova

What is the last resort washing solution for a pepsin column that has a blockage?

Recently my pepsin column got some blockage, supposedly because of the precipitation of aggregated protein. Multiple washes with 0.1% FA or pepsin wash (1.5M GuHCl + AcN + FA 95.2/4/0.8), as well...

21 April 2024 3,104 5 View

Is there a way to fix the following error in CGenFF: "attype warning: unknown sulfur type not supported;skipped molecule"?

Hello! The protein of my interest has a covalently bound inhibitor, which is a sulfone compound. I am currently trying to prepare all the files necessary for the MD simulation and use CGenFF...

04 September 2023 6,984 3 View