Elar Abd

5 Questions 7 Answers 0 Followers

Questions related from Elar Abd

What are the best cell lines to study the proliferation of neuronal cells?

I am studying on Parkinson's. I am using different compounds to stimulate the proliferation of neurons. I know stem cell is the best cell line to study for my research, but I would appreciate if...

03 March 2019 4,590 2 View

What are the best compounds to increase neuronal proliferation?

I am working with Parkinson cell lines ( SH-SY5Y ) and stem cells, I have to find compounds that increase proliferation of neurons. Thank you

01 January 2019 3,256 4 View

How can i optimize my cloning and screening?

I got my gene of interest in a vector which i do not know what it is. I got my gene through pcr and inserted into a known vector (PcDNA and Pgex2t), then i did digestion, ligation and cloned them...

05 May 2018 1,255 5 View

How can I design random primers to generate cDNA from mRNA?

I am going to make cDNA through Reveres Transcriptase PCR, I have to use random primers but I do not know how!

01 January 2018 9,627 6 View

What does happen when i overheat proteins in western blotting!

I prepared my samples for western blotting and I did overheat my samples mistakenly after adding sample buffer (SDS) for about 20 min, now i did western blotting but got no clear result. Can...

01 January 1970 3,160 4 View