Trypsin Digestion 0f 0.5/1.0 micro gram of proteins?

I have the following proteins in the mentioned formulation: pERK: 50 mM Tris-HCl, 150 mM NaCl, 0.25 mM DTT, 10 mM Glutathione, 0.1 mM EDTA, 0.1 mM PMSF and 25% Glycerol. PPARg: 50% Glycerol, 20 mM...

05 April 2017 1,966 3 View