Ekhlas Qaid Gazem

2 Questions 11 Answers 0 Followers

Questions related from Ekhlas Qaid Gazem

How can I identify the protein from excised gel of SDS PAGE band using LC MS/MS?

I've been trying to identify protein from SDS-PAGE (10% acrylamide gel). We digested a band around 25 kDa. we then sent the band for mass spectrometry identification, a lot of the proteins that...

12 November 2015 6,118 10 View

What is the purpose of using overlay gels containing ~8% acrylamide with 0.1% SDS instead of water?

Regarding to the protocol of pouring SDS-Polyacrylamide gels, at the step of "pour the acrylamide solution into the gap between the glass plates". We leave sufficient space for the stacking gel...

11 April 2014 5,345 4 View