Ekaterina M. Koltsova

3 Questions 3 Answers 0 Followers

Questions related from Ekaterina M. Koltsova

How to distinguish between unbinding and fluorophore quenching?

My system is made of a protein that binds to the membrane, which carries the epitope tag, and a nanobody, binding this tag. Membrane binding protein is a fusion protein consisting of a protein...

08 October 2024 2,389 2 View

How does the deprotonation of lysine occur before formaldehyde cross-linking?

Cross-linking of lysines with formaldehyde is a consequence of the nucleophilic attack of the formaldehyde carbon by the nitrogen of the epsilon-amino group of lysine. Normally lysine is...

04 October 2024 3,594 2 View

Ho can I calculate ionic strength of citrate buffer?

Hello, I have problems with calculating of ionic strength of citrate buffer. I intend to make 0.1 M buffer pH 6.0 containing 0.08 M sodium citrate (Na3C6H5O7 • 2H2O MW: 294.12 g/mol) and 0.02 M...

13 October 2023 2,742 0 View