Eira Choudhary

2 Questions 3 Answers 0 Followers

Questions related from Eira Choudhary

Can anyone suggest a good protocol for antibody supershift EMSA?

I am trying supershift in EMSA complex. I am using bacterial whole cell lysate, and antibody specific to my target protein (present in lysate). I am not able to pick supershift complex. Kindly...

08 August 2014 8,118 3 View

How can I perform EMSA with cell bacterial lysate to rule out nonspecific binding due to the presence of numerous other proteins, and then target one?

If the target DNA sequence can have binding specificity with any other random protein present in the lysate, then how to nullify this fact. Though, I have tried this EMSA with purified protein,...

03 March 2014 6,199 2 View