Einar Birnir Ólafsson

4 Questions 1 Answers 0 Followers

Questions related from Einar Birnir Ólafsson

What is the maximum cell density I can have dendritic cells (DCs) at for 2-24h and what is the lowest medium volume I can use for that number of DCs?

I am using DMEM + 10% FBS and 6 day old BMDCs in my experiment which is done using a 24 well plate. I will use the cells to make western blot samples, both cell lysates and supernatants will be used.

10 October 2014 9,271 3 View

Are there any MMPs that cannot degrade gelatin?

I am trying to detect MMP activity in BMDC total cell lysates and supernatants. This has proven to be more difficult than I anticipated. I am using fluorescein labeled gelatin (Enzchek Gelatinase/...

10 October 2014 9,168 4 View

Why no gelatinase activity?

I am trying to quantify the gelatinase activity of BMDC lysates. The BMDCs are incubated for 24h, the medium R10 is then removed and the cells are scraped in PBS, samples are then sonicated. I am...

10 October 2014 7,480 3 View

Can I precipitate the proteins in an R10 medium to concentrate my supernatant proteins?

I am looking for a protein secreted by BMDCs over 24h. I need allot of cells (2 * 10^6) so i also need allot of medium (i am using RPMI + 10% FBS (R10)). However, turns out my protein of interest...

10 October 2014 6,360 2 View