Ehasanullah Khan

3 Questions 5 Answers 0 Followers

Questions related from Ehasanullah Khan

Why no protein expression found in the same culture condition?

Hi, I am trying to express Trypanothione synthetase a protein from Leishmania, cloned in pET28a vector and transformed in E.coli. BL21 (DE3). The size of the protein is 74kDa. Initially, I...

22 May 2024 4,654 5 View

Why non specific amplification during colony PCR but no target amplification?

Hi! I hope you guys are doing well. I am doing colony PCR for confirmation of positive transformation. After colony PCR I got the multiple bands on agarose gel. The bands that appear on the gel...

29 September 2023 6,564 4 View

Why no amplification after gel elution and PCR with same primer set?

I am doing PCR to add a (GGGGS)4 linker at one end of a gene. After amplifying the gene with suitable forward and reverse primer, I got an excellent band on the gel, eluted it from the gel, and...

30 May 2023 5,135 4 View