Would it be feasible to run Exonuclease I and T7 exonuclease in a single pot reaction but have them work separately?

I am using Exonuclease I for its ss 3' - 5' exonuclease activity to clean up after second strand synthesis. I immediately follow up with T7 exonuclease digestion (ds 5'-3' exonuclease activity) to...

05 May 2021 9,666 0 View