Eduardo Marabuto

3 Questions 7 Answers 0 Followers

Questions related from Eduardo Marabuto

Retrieving good quality DNA from old insect specimens?

What are the best protocols / extraction kits for retrieving DNA out of old insect specimens? I am on the way to attempt extracting mitochondrial and nuclear DNA from some rather old, dry insect...

05 May 2016 5,180 1 View

Has anyone used PERMUT for genetic diversity studies?

Hi all. I have population genetic diversity data (mtDNA) and am trying to use the software PERMUT (http://www.pierroton.inra.fr/genetics/labo/Software/Permut) as described theoretically by Pons...

07 July 2014 4,468 7 View

How to anneal long oligos (~80bp) with high GC content (and produce sticky ends upon annealing)?

I have tried mixing them in 1x Annealing Buffer (10 mM Tris, pH 7.5–8.0, 50 mM NaCl, 1 mM EDTA) at 10uM final concentration for both the forward and the reverse and then putting them at 97ºC for...

02 February 2013 391 24 View