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I want to mimic exercise conditions in vitro.
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I researched on the internet question above, I couldn't find any directing site. In general, scientists had isolated their own BAT precursor ( Myf5+) and Brown adipocyte cells in their...
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I know one miRNA regarding my study. On the other hand I think this miRNA is blocked by the another miRNA which I don't know when the conditions changed. İs there any method to identify the new...
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As you know, 16S rRNA is used to in phylogenetic classification owing to slow rates evolution of this gene, being in all prokaryotes etc. But which mechanism maintains this situation? Or is...
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In total, I have a gene which has 896 bases. After DNA sequencing process (as I mentioned in the title), approximately both are on the beginning portion. Almost 100 bases at the beginning portion,...
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First when I did PCR with a new primers I saw many bands on agarose. I adjusted annealing temperature for this PCR process, 50 degree. Subsequently, I prepared new PCR mixture from new cDNAs that...
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Because I have bright bands in the onset of well of agarose gel. I have a doubt about contamination. I used a control cDNA to verify PCR process. I attained the right Dna fragments as I expected....
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