Edgard Francisco Jauregui

4 Questions 3 Answers 0 Followers

Questions related from Edgard Francisco Jauregui

Does anybody know a good protocol for Mycorrhiza colonization in Medicago truncatula?

I am currently working to introduce this technique in my lab. I was wondering whether someone can help me with some suggestions and protocols, that have a good consistency obtaining arbuscules...

07 March 2016 2,671 3 View

BNM and Fahraeus media?

I am a little confused. Are BNM (Buffered nod media) and Fahraeus media the same?. I am trying to induce nodulation and which of them is the best media to produce nodules. In case you know a...

07 March 2016 7,521 0 View

Why my ligation is not working with a Gateway final vector (12Kp) and small insert (0.3Kb) ?

I am currently working with a final destination vector (gateway technology) that I want to replace a gene native promoter and replace it with CaMV35S promoter. I have cut my vector (pKDWG2R) with...

03 September 2015 10,060 3 View

Does anyone have experience with GDNA extraction-Medicago truncatula?

I currently working with M. truncatula and I am trying to perform some gDNA extraction out of leaf tissue. I have been using the following extraction buffer: 200mM Tris,pH7.5, 250mM NaCl, 25mM...

30 July 2014 9,452 3 View