Edappayil Janeeshma

5 Questions 10 Answers 0 Followers

Questions related from Edappayil Janeeshma

How can I detect isoelectric point of a protein?

With acetic acid serial dilution i take an effort to detect the isoelectric point of an enzyme. I observed precipitation in PH 2.85 and 3.1.Of them which one want to taken as isoelectric point?plz...

01 January 2017 4,660 9 View

How can i determine positional specificity of lipase with tlc

i would like to focus on the substrate and positional specificity of lipase frompseudomonas

11 November 2016 4,554 3 View

How can i calculate activity of immobilized lipase ?

i have to calculate activity of immobilized (using sodium alginate) lipase using pNPP assay

09 September 2016 7,418 12 View

What is the protocol to isolate dna from gordonia ( gram positive bacteria)

i tried to isolate dna from gordonia using different method (lyzozyme treating, heating). but not get resault

08 August 2016 8,825 2 View

On repeatation of pcr reaction i did not get the pcr product under same condition. Is it possible?

i did pcr amplification of lipase gene. but when i tried to repeat the experiment i did not get a band. i checked the dna concentration in nanodrop it is 1000ng/microlitre. i did the reaction in...

08 August 2016 6,576 8 View