Echo Zhu

5 Questions 3 Answers 0 Followers

Questions related from Echo Zhu

Why don’t primary microglial cells adhere to T25 flasks and plates?

I digested brain tissue with 0.25% trypsin at 37°C for 30 minutes, then isolated microglial cells using 30% Percoll gradient centrifugation. I cultured the cells in 5% MEM or DMEM medium, but the...

13 January 2025 7,959 3 View

Why not use CD11b staining to evaluate the purity of primary microglia?

I have noticed that, regardless of whether isolating primary microglia from humans or mice, purity is generally assessed using CD11c flow cytometry staining. Why not use CD11b instead?

03 July 2024 9,477 2 View

How to perform macrophage-T cell co-culture?

Hello everyone. I am researching the influence of CD8+ T cells on macrophage polarization in vitro. But I don't know how to co-culture them, e.g. plates, cell concentration, culture time, etc. I...

15 September 2022 3,156 1 View

What's the difference between rhMOG and rmMOG induced EAE model?

I noticed that researchers use MOG35-55 to induce a type of T cell-dependent EAE, while for both T cell and B cell-dependent EAE, they choose rhMOG. Some papers report that B cells also contribute...

06 July 2022 4,393 1 View

Why rituximab can not deplete B cells in mice?

Rituximab is a B-cell-depletion monoclonal antibody that is used to treat rheumatoid arthritis and other diseases. The chimeric structure of rituximab comprises human IgG 1 and kappa-chain...

20 August 2021 2,493 2 View