Ece Cagdas

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Questions related from Ece Cagdas

Has anyone cloned shorter plasmid than expected?

I have been cloning TOPO vector containing 3'UTR of my gene of interest into pmirglo vector (7350 bp). I digested pmirglo and topo vector with XhoI and SacI and ligated them. I also digested...

15 May 2018 8,956 3 View

Has anyone cotransfected a plasmid containing 3'UTR of gene of interest and another plasmid expressing miRNA sequence to HEK293T cells?

I cotransfected HEK293T cells with a plasmid containing 3'UTR of gene of interest and another plasmid containing miRNA sequence. I used equal amount for these two plasmids. After I did luciferase...

25 December 2017 5,976 1 View

Has anyone use infusion cloning to insert short DNA into a vector?

Hi, I want to insert 130 bp DNA into 3000 bp vector, however I could not obtain colonies. I used 50 ng insert DNA and also tried to increase insert amount but still I could not observe any...

11 November 2016 3,320 5 View