ec Sener

3 Questions 18 Answers 0 Followers

Questions related from ec Sener

Any tips on storing protein at -80?

Hello, So I have sonicated some bacteria culture and gathered protein cell debris mix. I want to perform SDS Page and some chromotography procedures a few months-years later from these very...

06 June 2016 6,235 11 View

Ever used SDS Page Precast Gels (mini protean vs criterion) ?

Hello I am planning to perform SDS Gel electrophoresis and my protein is about 6kb. I was checking on BioRad and saw many kinds of precast gels. 1) How should i decide whether to use Tris Hcl or...

05 May 2016 6,789 5 View

Why would you do subcloning when you can put your insert to an expression vector directly?

Hi, Here I explain my question more. Im a molecular biologist in struggle. I have a procedure and there are 2 different vectors derived from Ecoli and and an insert which will be put into an Ecoli...

05 May 2016 4,330 4 View