Ebubekir Ayhan

4 Questions 4 Answers 0 Followers

Questions related from Ebubekir Ayhan

How do you dissolve acetone precipitated antimicrobial peptides?

Hello everyone, I am trying to isolate antimicrobial peptides from plants. After precipitating the peptides with acetone, most of the pellet (peptides) did not dissolve. I used dH2O/PBS to...

15 December 2023 9,108 2 View

After staining DNA with Etbr, can silver staining be performed on the same sample?

I am studying DNA-ligand interactions with EMSA. Sometimes I cannot see the DNA bands in the PAGE gel when I stain it with Etbr. As silver staining is more sensitive to EtBr, I have to use the...

01 November 2023 6,048 2 View

What are the causes of not increasing amper in Non-denaturing PAGE?

Hello everyone, I wanted to visualize my DNA fragments after PCR in Non-denaturing PAGE, however I could not. Because amper was not increased during the PAGE assay. what are the causes of not...

03 November 2022 7,146 4 View

Is there any alternative method to isolate antimicrobial peptides from plants without using protease inhibitor cocktail ?

Hello everyone, I want to isolate antimicrobial peptides from plants but, I don't have a protease inhibitor cocktail right now and possibly not in the near future. Is there any alternative...

29 August 2022 5,020 3 View