Eason Zhang

2 Questions 5 Answers 0 Followers

Questions related from Eason Zhang

Unexpected self-ligated results ?

Hi, hope you're doing well in your research. I have been doing restriction enzyme digestion and ligation to construct plasmids for two weeks and I got very unexpected self-ligated plasmids. The...

12 February 2023 9,077 5 View

Troubleshooting for overlap extension PCR ?

Hi everyone, I am currently trying to splice two DNA fragments together by OE-PCR. I have one fragment which is around 1400 bp and the other is around 100bp. The two fragments have 20 bp in...

24 October 2022 3,438 6 View