Dziedzom Komi de Souza

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Questions related from Dziedzom Komi de Souza

Can someone advise on the gene copy number determination using the delta-delta Ct method?

Can anyone give me a simple way (step by step) of calculating the copy number of a gene, using a reference gene (S7) for normalization? I run the RT-PCR and obtained my Ct values. I think I...

30 September 2013 4,238 11 View

Can anyone give me a step-by-step procedure of how to get rid of stop codons from sequences to be submitted to GenBank?

Can anyone give me a step-by-step procedure of how to get rid of Stop Codons from sequences to be submitted to GenBank? My first attempt resulted in frameshifts that reintroduced other stop...

05 December 2012 4,801 22 View